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KMID : 0380619930250010015
Korean Journal of Food Science and Technology
1993 Volume.25 No. 1 p.15 ~ p.21
Studies on Rheological Characterization of Barley ¥â - Glucan [mixed - linked(1 - 3) ,(1 - 4) - ¥â - D - Glucan]



Abstract
Crude [3-glucan extracted from Barley was purified by stepwise enzyme treatment (Thermostable a-amylase, amyloglucosidase, protease). The Intrinsic Viscosity [q] of the purified (3-glucan was determined by Cannon Fenske Capillary Viscometer (size 50, Cannon Lnstruments, State, College pa.) at different pH (2,4,7,9,11) and various salt concentration (0.01 M, 0.03 M, 0.05 M, 0.07 M, 0.1 M and 0.2 M). The [rl] of purified (3-glucan was ranged from 0.997^-2.290 dl/g. The [rl] of purified (3-glucan at both alkali, acid condition were lower than those at pH 7. However, the alkali condition of puified (3-glucan solution showed less [rl] than the acid condition of this solution. From 0 M to 0.2 M salt concentration, the [rl] of purified (3-glucan solution was decreased to 0.03 M then increased to 0.05 M NaCl and remained constant to 0.2 M NaCl. The chain stiffness parameter of purified (3-glucan was not affected by temperature from 15¡É to 65¡É. The shear rates of various (3-glucan conditions were determined by Bohlin Rheometer (Lund, Sweden). The (3-glucan concentration of 1.0 g/dl and 2.0 g/dl behaved as Newtonian fluid. However, above the concentration of 3.0 g/dl (3-glucan solution, it showed thixotropic and psedoplastic characteristics. Barley (3-glucan appears a damping at 0.5 frequency for the 4.0 g/dl solution. Below 0.5 frequency, it appears a viscous behavior property and above 0.5 frequency, it appears a elastic behavior property.
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